Promethease Review & Tutorial/Tour
Hi guys! It’s Petrina, your go to girl for
learning how to make fit work in a crazy busy world and today’s video is going to be a follow
up to last week’s where I reviewed 23andme and in this one, I’m gonna show you how to
use Promethease, which is a very cool $5 tool that will dig deep into your raw data that
you got from the 23andme test or wherever you had your DNA tested and this will give
you all sorts of really cool medical info based on your data. Now before I take you
in the platform, I want to start you off with big fat disclaimers so pay attention! Go ahead
and put your listening ears on people. The information you get on Promethease is not,
I repeat not a medical diagnosis so don’t use it as such! Note that just because you
have an increased risk for something doesn’t mean you’re gonna get that something so calm
the (bleep) down! Chillax people! Capiche? Now having got that out of the way, let’s
go on in and I’ll give you a big tour. Alright so here we are on the Promethease platform.
This is what you’re gonna see after you pay your $5 and you check all the boxes they give
you for legal approvals and you get your raw data uploaded and processed and I’m gonna
remind you, Don’t Freak Out! I know it looks like a hot mess of technical data and you’re
likely gonna see some bad news pop up right away, like this little gem on coronary artery
disease for me personally. Note too that you’re gonna see that kind of stuff probably because
they assign bad things a higher magnitude level, so just take a deep breath and know
that you’re gonna filter, filter, filter to get the most relevant and most interesting
stuff that’s for you. As you see too, when you first get in you’re
gonna have a boatload of associations showing up. As you can see here there are 19,139 rows
on mine and that’s a lot of information. It would take me a long, long time to go through
all 19,000 and if you have that kind of time on your hands, go for it, but if you’re like
me and time crunched and you want to find the most relevant and interesting info, you’re
going to filter. Now you’re gonna see too you can sort by topics and there’s gonna be
all sorts of different ones. You can scroll down if you are just interested in a search
group topic like eye color. You can see the bits of information that’s related to that
topic by going that way. You can also do it by medicines, medical conditions, ClinVar
Diseases, your genes and as you’ll see too when you upload your information you’ll have
the option to pic a race that you’re in. You don’t have to pick, but if you do, you’re
gonna be able to learn how frequent your gene variation is within your pool of those in
that same race. Mine is Caucasians from Northern and Western Europe and if you look on the
frequency tab for different pieces of information you’ll see a percentage and that means that
particular variant is present in just 20% of people in that race. If you’re interested in one specific thing
already you can just go in that search tab and type it in and as you’ll see, you can
choose to show information on SNPs, Genosets or both. SNPs are single nuclear polymorphisms
and Genosets, well rather than bore and confuse you with all the details, I just say to have
both selected when you are filtering. Repute is just like you’d imagine. The reputation
of the association, whether it be good, bad or not set. I will say too that just because
something is, the repute is not set it doesn’t necessarily mean that it’s not good or not
bad. It could be that someone just hasn’t assigned a certain repute to that particular
piece of information you’re looking at. All of the pieces of information that you see
are compiled in SNPedia which is sort of the Wikipedia of DNA information that’s heavily
reliant on users going in and you know putting information in so just because something is
not set doesn’t mean that it is not good or bad. It could be that it just hasn’t been
set yet. I’m gonna go ahead and keep all checked, but if you just want to see the good or you’re
a pessimist and just want to see the bad you can limit it that way. Magnitude is a really good indicator of just
how interesting the information is likely to be and note that this is subjective. This
is again based on certain people who assign magnitudes to it when they’re going through
data. When you hover over it, you’ll see that it says 0 is boring, 1 is unset or 2+ is interesting.
So in order to filter down my results to be a more manageable level to review, I’m gonna
go ahead and set mine to 2. This way I’m gonna weed out the stuff that’s not likely to be
that important in the grand scheme of things and note too that even though the scale that
you can set to filter goes up to 4. You could find some things that are listed all the way
up to 10. Like let’s say the BRCA 1 is associated with breast cancer. That’s a 10. Denys-Drash, Frazier, Raine syndromes are all 9s as are early onset Alzheimer’s Disease. References are gonna indicate how well researched
certain findings are. I wouldn’t go less than 2 for credibility purposes for mine because
I’d like to see things that are more credible. I’m gonna set it at 12. You can set it again
at whatever number you like. If you want it to be really credible you can go higher. If
you want to see more things you can go lower. It’s all up to you and if you wanted to see
only items where the SNP or the Genoset variation is rare for your ethnicity type, you could
also filter by frequency. The lower the number, the rarer the variation is gonna be. For this
demo I’m not gonna filter by frequency or require a certain level, but if you wanted
to again that’s up to you. Now the UI is your user interface and it defaults
to the tool tips. If you’re someone who wants to go in and get really geeky and contribute
to SNPedia you can use the editor mode where you’d be able to edit certain things and make
certain classifications. I’m gonna leave it as tool tips. So in moving that magnitude
to 2 and the reference is a 12, you’ll see those 19,000 results that I had in the beginning
got reduced to a far more manageable 104. Now if you like to cheat and see all that’s
come up after you’ve filtered, you can kick back to times more button until you got up
to the total. Okay so once filtered and you’re ready for
review, note that the boxes that you see that are outlined in red are those that have been
classified as having a bad repute, a bad reputation. The green ones are ones with a good repute
and gray are ones that haven’t been set. You’ll also notice highlighting in the frequency.
There’s always a color and the darker the red, the rarer the variation is for your particular
ethnicity. Now too as I said at the beginning, it’s gonna sort firstly based on magnitude
so the higher magnitude stuff is gonna be at the top of that list after filtering and
it will go down to lower as you scroll down and because usually our interest level is
peaked by bad things more so than good, I think the bad stuff are gonna have a higher
magnitude so you’re gonna be greeted with some bad news once you get in and that’s okay.
Again it doesn’t mean it’s a diagnosis. It doesn’t mean you’re actually going to get
it. Like here the top one for me is the coronary artery disease so don’t freak out and if you’re
concerned about certain things you know talk to your doctor. Make good lifestyle choices. As you’ll see, with most entries at the bottom,
you can even click on different topics that come up if you want to learn more. The medicines,
there’s also a medical conditions category, medicines or a ClinVar to learn more about
a particular topic or if you want to keep it to learning more about the particular study
that they’re referencing, you can click on that Rs link at the top to learn more. It
will take you to the SNPedia version of it where it has like an abstract of what the
study is and it will also have PMIDs where you can possibly see abstracts or if you can
see the OA designation you can see the full article online if it’s something you want
to learn more about. I want to warn you too if you are starting
to dig and clicking on the different tabs to learn a little bit more, you’re going to
have to reset to get back to the filtered results after you go back to Promethease.
That can be a frustrating part in using this system, but I wanted you to know just in case
you wanted to click around that when you go back, if you want to get it back to that filter
you’re gonna have to go through and filter all over again. I wanted to note too as you
can see with this that intermediate metabolizers, the most potential useful information I think
you can get from Promethease is that relates to medicines. It’s really useful to know how
certain medications can impact you, whether it be the metabolization rate benefits, the
odds of you responding to certain drug treatments, you can find that out by going through and
going through your results. It’s really good to go through these and find
credible studies and if you’re concerned about certain things, share them with your doctor
and you’ll sometimes see in these abstracts of certain studies that there are ways to
minimize your risk for certain things that come up like here for age related macular
degeneration it notes that I could eat a diet richer in vitamins C and E, lutein, zeaxanthin
and the minerals zinc and copper as to reduce the risk. You can also learn not physical
related, but mental and attitude. This is a study on optimism and empathy. Again, it
ain’t all about panic mode and scary diseases. You can find other little interesting tidbits
of information, including this gem – that caffeine will not make your breasts smaller.
Learning is fun! And that’s it for my Promethease overview.
I hope you found it tremendously helpful. I know it can be really intimidating getting
on the system, but once you know what everything means and how to filter it to get the most
relevant and interesting information it can be a really powerful tool. As always, if you have any questions feel
free to shoot me an email at [email protected] If like, check out my crazy sexy cool website
over at PetrinaHammFitness.com. If you like me are feeling the impact of Jonah stay safe,
keep warm and have a great weekend. Bye guys!
Great video! Thanks for finding my video and leading me to your channel! I will have to log into Promethease when I'm not at work 🙂
I finally got my results back from stupid 23andme, which didnt tell me crap. I submited it to Promethase and i got way more details. apparently I'll live to over 100 but my prostate is fucked..
FYI, if you right click when you look at the SNP, right click it and open it in a new tab, you'll still have the old tab so you don't have to reset it every time, which drove me crazy too. Great video. Thanks
I had this also pop up on my very first slide. rs1333049(C;C)1.9x increased risk for CAD think thats what you had…
I had way too many thing contradict themselves, often. Some different results that say things about the same thing, that make little scene. Soo very confused.
I'm having trouble uploading results from familytreedna , if you have any insights as to how that's done please let me know when you have a moment , Petrina. Nice videos , helpful & informative *
So if I am reading the Frequency correctly for increased risk for breast cancer 7.1% of people with your same genetic makeup had the gene for breast cancer? This is so very interesting and could truly help the medical field if we all had these tests done. Not just for cancer, but for tons of conditions.
Once you've downloaded the report, is there a way to print it? Maybe load it into a spreadsheet program? Or do you always have to click on the downloaded html file and go back on the web to review the report?
In the PGx tab everything is shades of green or white for those that weren't tested but I have one that is red 89 MTHFR RS1801131 BAD 2.1 possibly impaired Folate metabolism. Sadly its spot on as stroke runs in the women in my family.
Does Promethease provide information about methylation genes> Or carb, fat metabolism? Enzymatic mutations?
How do you access the download from Promethease using an Apple Mac (MacBook)?
I can only view it online (runs out in 45 days) at the moment. Thanks in advance.
you can also take your 23 and me data and up load to the site Dna.land this site will use "imputation" for your data and give better and more info and then re upload for $10 to Promethease. Very cool.
Thanks for the tutorial. It was more helpful than the ones that Promethease suggests. An FYI for you though, SNP is pronounced "snip" 🙂
loved this. thanks
Question, under the "medical conditions" tab there are numbers "(110)" listed next to some of the conditions. What does this mean? Many Thanks in advance.
Thank You for walking us through, makes reading the report such a breeze.
Super helpful video 🙂 Thanks!
Thanks for the knowledge, sister! So you can upload the .csv to the Promethease site?
music makes it very difficult to understand but information is excellent. Ditch the music
Great information! It would be even better without the music.
Thanks for such a helpful video.
At the risk of sounding stupid, I thought Promethease also revealed your ancesty results – or did I get that bit wrong?
One thing your forgot to mention is the risk may not be necessarily on you, it could be to your possible offspring. That's something worth noting.
I am a female and Promethease estimated two markers for my "Y DNA". Is this accurate?
Thanks, this was very helpful and clear for a beginner.
Cost me $10
Hello, how can the men find their haplo group?
I do not understand what you are saying about the "Repute" tab. Does it mean that the reputation of the test/or result is bad, or having this gene is bad?
Oh, and the test cost $10 now
This video is the best I've come across so far! my blog post https://www.watersheddna.com/blog-and-news/filtering-a-promethease-report-one-genetic-counselors-strategy adds by explaining why there is the "not medical information" disclaimer on the Promethease site and reports.
Great video! I have ADHD so the fun music actually helped me stay tuned to boring DNA tutorial info!
Hello Petrina, Today, I uploaded both my wife and my ancestry files and have the promethease results. My question is, on the second line, if it says "0" Magnitude.. what exactly does that mean?
You did an excellent job on this video. I reluctantly clicked on it and thought I wouldn't watch it to the end, but you did a fantastic job in a relatively short period of time describing the Promethease web app, how to set it up and some of the benefits along with the downfalls. I may even check out some of your other videos along with some of your links. For the record I'm a bald, grumpy old guy who is not part of the target demographic you are trying to appeal to. But you did a great job and I thought you should know that. I have also developed a severe hearing impairment since heart surgery and I did not find the music distracting at all.
Thank you for making this. I opened up my report and instantly went to Youtube to understand the findings. Appreciate your help!
Just a FYI. I had my entire genome sequenced including mitochondrial and Y-chromosome through national geographic. Promethease uploaded it just fine. I understand that ancestry and 23-and-me just sequence snippets. I think that your entire genome would be better analyzed on Promethease.
Music is too loud and very distracting. I'm quitting at 4:03. It's a wonder you didn't ditch the music 2 years ago when it was suggested.
Nicely done. If you could re-post this without the distracting music that would be amazing.