Promethease Review & Tutorial/Tour

Hi guys! It’s Petrina, your go to girl for
learning how to make fit work in a crazy busy world and today’s video is going to be a follow
up to last week’s where I reviewed 23andme and in this one, I’m gonna show you how to
use Promethease, which is a very cool $5 tool that will dig deep into your raw data that
you got from the 23andme test or wherever you had your DNA tested and this will give
you all sorts of really cool medical info based on your data. Now before I take you
in the platform, I want to start you off with big fat disclaimers so pay attention! Go ahead
and put your listening ears on people. The information you get on Promethease is not,
I repeat not a medical diagnosis so don’t use it as such! Note that just because you
have an increased risk for something doesn’t mean you’re gonna get that something so calm
the (bleep) down! Chillax people! Capiche? Now having got that out of the way, let’s
go on in and I’ll give you a big tour. Alright so here we are on the Promethease platform.
This is what you’re gonna see after you pay your $5 and you check all the boxes they give
you for legal approvals and you get your raw data uploaded and processed and I’m gonna
remind you, Don’t Freak Out! I know it looks like a hot mess of technical data and you’re
likely gonna see some bad news pop up right away, like this little gem on coronary artery
disease for me personally. Note too that you’re gonna see that kind of stuff probably because
they assign bad things a higher magnitude level, so just take a deep breath and know
that you’re gonna filter, filter, filter to get the most relevant and most interesting
stuff that’s for you. As you see too, when you first get in you’re
gonna have a boatload of associations showing up. As you can see here there are 19,139 rows
on mine and that’s a lot of information. It would take me a long, long time to go through
all 19,000 and if you have that kind of time on your hands, go for it, but if you’re like
me and time crunched and you want to find the most relevant and interesting info, you’re
going to filter. Now you’re gonna see too you can sort by topics and there’s gonna be
all sorts of different ones. You can scroll down if you are just interested in a search
group topic like eye color. You can see the bits of information that’s related to that
topic by going that way. You can also do it by medicines, medical conditions, ClinVar
Diseases, your genes and as you’ll see too when you upload your information you’ll have
the option to pic a race that you’re in. You don’t have to pick, but if you do, you’re
gonna be able to learn how frequent your gene variation is within your pool of those in
that same race. Mine is Caucasians from Northern and Western Europe and if you look on the
frequency tab for different pieces of information you’ll see a percentage and that means that
particular variant is present in just 20% of people in that race. If you’re interested in one specific thing
already you can just go in that search tab and type it in and as you’ll see, you can
choose to show information on SNPs, Genosets or both. SNPs are single nuclear polymorphisms
and Genosets, well rather than bore and confuse you with all the details, I just say to have
both selected when you are filtering. Repute is just like you’d imagine. The reputation
of the association, whether it be good, bad or not set. I will say too that just because
something is, the repute is not set it doesn’t necessarily mean that it’s not good or not
bad. It could be that someone just hasn’t assigned a certain repute to that particular
piece of information you’re looking at. All of the pieces of information that you see
are compiled in SNPedia which is sort of the Wikipedia of DNA information that’s heavily
reliant on users going in and you know putting information in so just because something is
not set doesn’t mean that it is not good or bad. It could be that it just hasn’t been
set yet. I’m gonna go ahead and keep all checked, but if you just want to see the good or you’re
a pessimist and just want to see the bad you can limit it that way. Magnitude is a really good indicator of just
how interesting the information is likely to be and note that this is subjective. This
is again based on certain people who assign magnitudes to it when they’re going through
data. When you hover over it, you’ll see that it says 0 is boring, 1 is unset or 2+ is interesting.
So in order to filter down my results to be a more manageable level to review, I’m gonna
go ahead and set mine to 2. This way I’m gonna weed out the stuff that’s not likely to be
that important in the grand scheme of things and note too that even though the scale that
you can set to filter goes up to 4. You could find some things that are listed all the way
up to 10. Like let’s say the BRCA 1 is associated with breast cancer. That’s a 10. Denys-Drash, Frazier, Raine syndromes are all 9s as are early onset Alzheimer’s Disease. References are gonna indicate how well researched
certain findings are. I wouldn’t go less than 2 for credibility purposes for mine because
I’d like to see things that are more credible. I’m gonna set it at 12. You can set it again
at whatever number you like. If you want it to be really credible you can go higher. If
you want to see more things you can go lower. It’s all up to you and if you wanted to see
only items where the SNP or the Genoset variation is rare for your ethnicity type, you could
also filter by frequency. The lower the number, the rarer the variation is gonna be. For this
demo I’m not gonna filter by frequency or require a certain level, but if you wanted
to again that’s up to you. Now the UI is your user interface and it defaults
to the tool tips. If you’re someone who wants to go in and get really geeky and contribute
to SNPedia you can use the editor mode where you’d be able to edit certain things and make
certain classifications. I’m gonna leave it as tool tips. So in moving that magnitude
to 2 and the reference is a 12, you’ll see those 19,000 results that I had in the beginning
got reduced to a far more manageable 104. Now if you like to cheat and see all that’s
come up after you’ve filtered, you can kick back to times more button until you got up
to the total. Okay so once filtered and you’re ready for
review, note that the boxes that you see that are outlined in red are those that have been
classified as having a bad repute, a bad reputation. The green ones are ones with a good repute
and gray are ones that haven’t been set. You’ll also notice highlighting in the frequency.
There’s always a color and the darker the red, the rarer the variation is for your particular
ethnicity. Now too as I said at the beginning, it’s gonna sort firstly based on magnitude
so the higher magnitude stuff is gonna be at the top of that list after filtering and
it will go down to lower as you scroll down and because usually our interest level is
peaked by bad things more so than good, I think the bad stuff are gonna have a higher
magnitude so you’re gonna be greeted with some bad news once you get in and that’s okay.
Again it doesn’t mean it’s a diagnosis. It doesn’t mean you’re actually going to get
it. Like here the top one for me is the coronary artery disease so don’t freak out and if you’re
concerned about certain things you know talk to your doctor. Make good lifestyle choices. As you’ll see, with most entries at the bottom,
you can even click on different topics that come up if you want to learn more. The medicines,
there’s also a medical conditions category, medicines or a ClinVar to learn more about
a particular topic or if you want to keep it to learning more about the particular study
that they’re referencing, you can click on that Rs link at the top to learn more. It
will take you to the SNPedia version of it where it has like an abstract of what the
study is and it will also have PMIDs where you can possibly see abstracts or if you can
see the OA designation you can see the full article online if it’s something you want
to learn more about. I want to warn you too if you are starting
to dig and clicking on the different tabs to learn a little bit more, you’re going to
have to reset to get back to the filtered results after you go back to Promethease.
That can be a frustrating part in using this system, but I wanted you to know just in case
you wanted to click around that when you go back, if you want to get it back to that filter
you’re gonna have to go through and filter all over again. I wanted to note too as you
can see with this that intermediate metabolizers, the most potential useful information I think
you can get from Promethease is that relates to medicines. It’s really useful to know how
certain medications can impact you, whether it be the metabolization rate benefits, the
odds of you responding to certain drug treatments, you can find that out by going through and
going through your results. It’s really good to go through these and find
credible studies and if you’re concerned about certain things, share them with your doctor
and you’ll sometimes see in these abstracts of certain studies that there are ways to
minimize your risk for certain things that come up like here for age related macular
degeneration it notes that I could eat a diet richer in vitamins C and E, lutein, zeaxanthin
and the minerals zinc and copper as to reduce the risk. You can also learn not physical
related, but mental and attitude. This is a study on optimism and empathy. Again, it
ain’t all about panic mode and scary diseases. You can find other little interesting tidbits
of information, including this gem – that caffeine will not make your breasts smaller.
Learning is fun! And that’s it for my Promethease overview.
I hope you found it tremendously helpful. I know it can be really intimidating getting
on the system, but once you know what everything means and how to filter it to get the most
relevant and interesting information it can be a really powerful tool. As always, if you have any questions feel
free to shoot me an email at [email protected] If like, check out my crazy sexy cool website
over at If you like me are feeling the impact of Jonah stay safe,
keep warm and have a great weekend. Bye guys!


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